NIH/3T3-CAS9 cell line

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NIH/3T3-CAS9 Order

NIH/3T3-CAS9 cell line
Catalog#
YC-A013-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
NIH/3T3-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
NIH/3T3-CAS9
Cell Morphology
Fibroblast-like, adherent
Passage ratio
1:3~1:5
Species
Mouse
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the NIH/3T3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a NIH/3T3-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
NIH/3T3-CAS9 Cas9 16.91583252 3.60078049
NIH/3T3-CAS9 β-actin 13.31505203
NIH/3T3 Cas9 33.85106277 20.10673046
NIH/3T3 β-actin 13.74433231
Cutting Efficiency Validation
NIH/3T3-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the NIH/3T3-CAS9 stable cell pool which is electroporated by Tmem65 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NIH/3T3		 STR Info (Cell bank)
Cell Line:NIH 3T3
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 19.320.319.320.3
6-7 12.012.0
19-2 10.011.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.010.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.015.017.0
17-2 13.014.013.014.0
12-1 20.020.0
5-5 14.015.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
NIH/3T3-CAS9 cell line
NIH/3T3-CAS9
Catalog#
YC-A013-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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